DEPT (Distortionless Enhancement by Polazization Transfer) is a 13C detected, multiplicity analysis experiment that differentiates CH, CH2 and CH3 carbons from each other. Quaternary carbons are suppressed in the experiment. Varian supplies a single DEPT pulse sequence (Dept). The editing is done in the pulse sequence via a 1H pulse set to a 45, 90, or 135 degree rotation. The actual setting is through a multiplier mult to a 90 degree pulse as shown in the table below:

Rotation (degree) mult Edited Results
45 0.5 CH+CH2+CH3 (up)
90 1.0 CH (up)
135 1.5 CH+CH3 up (positive); CH2 down (negative)

In the following, as commonly found in literature, we will refer to the individual elements of dept as dept45, dept90 and dept135.

Varian Dept Pulse Sequence

Varian DEPT Pulse Sequence


Procedure (on NMR500 with SWP probe)

Notes for all DEPT experiments:

Option 1 (recommended)

Helpful Processing and Printing Commands for DEPT

Option 2 (For individual dept45, dept90, or dept135 runs)

Default parameters


  • Sample: Strychnine at ~ 25mg/mL (~ 100mM) in cdcl3
  • Data collected October 2010 (Option 1, ~9 mins with nt=64)
The 4 elements of Dept with mult=0.5, 1, 1, 1.5 (from bottom to top) correspond to dept45, dept90, dept90, and dept135. These raw dept elements are sufficient for multiplicity analysis. Further processing with adept is optional. raw dept
Spectra further processed with adept command. From top to bottom:

1: CH3

2: CH2

3: CH

4: All (CH+CH2+CH3)


H. Zhou updated Oct 2010