2D Gradient Enhanced COSY

The following figure is the gradient based 2D COSY pulse sequence with the main parameters displayed (NMR500).

Gradient COSY Sequence

Procedure (on NMR600)

Default parameters

The default setting (modified from Varian's default values) in our parameter set uses the following for a quick 2D COSY:

Data Processing

You can use vnmrJ to process the data, but vnmrJ's graphics display for 2D spectrum leaves much to be desired. A separate program is strongly recommended for detailed processing and analysis. Mnova and nmrPipe/nmrDraw are installed on NMR600 and the data processing station.

NOTE: do not phase any absolute-value or magnitude mode spectrum such as COSY or HMBC setup here.

Example spectrum

2D absolute-value COSY

Expansion of the 1.0ppm to 4.7ppm region:


H.Zhou updated Apr 2012