1D and 2D NOESY Comparison

1D sequence: Gradient enhanced NOESY1D

2D sequence: Gradient enhanced 2D NOESY

Sample: Strychnine at ~ 25mg/mL (~ 100mM) in cdcl3
  • Data collected October 2010
  • Processed and displayed with nmrPipe/nmrDraw

1D NOESY

  • ~5 mins each experiment, with 64 scans
  • red cursor line marks excitation position

2D NOESY

  • ~15-30 mins with 4 scans per increment
 Strychnine

Strychnine

1D NOESY Spectra

Selective inversion of the peak at ~7.2ppm (~ 5 mins). Note the imperfect selectivity of the excitation pulse excited an adjacent peak to the left which has NOE to the resonance at 8.2ppm. Also, note small residual artifact peaks across spectrum. The genuine NOE peaks appear at ~ 1.9ppm and 3.95ppm.

noesy1d

Selective inversion of peak at ~5.95ppm (~ 5 mins). NOE peaks appear at ~4.2ppm and 2.7ppm.

noesy1d

2D NOESY Spectrum

The 2D spectrum offers much better clarity and sensitivity than 1D NOESY.

15 mins experiment with ni=75. Linear prediction is applied along indirect dimension.

15 mins NOESY

30 mins experiment with ni=150. Linear prediction is applied along indirect dimension.

2D NOESY

H. Zhou updated Sept 2010