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NMR Theory and Techniques

Note: During training and assisting students and researchers, I often find it helpful to go over some NMR theory which is usually picked up in bits and pieces sporadically over the years for most users. Evidently, this work has evolved into a much bigger project in a short time. I hope these materials will spur more interest in NMR and help all users, especially new ones, to broaden their skills and understanding of the NMR techniques. Some data shown here are synthesized, perfect data for illustration purposes. The data are generated with in-house written Tcl/Tk scripts, converted to nmrPipe binary data, and then processed with Mnova. Many common NMR terminologies are highlighted in bold. Since other aspects of the NMR techniques are covered in various lab classes, focus is given here to better understanding the mathematical and physical basis of various NMR observations and techniques. Some are very relevant to routine practice, especially in the design of NMR experiments and data interpretation. -- Hongjun Zhou, @UCSB, 01/2019
"I insist upon the view that 'all is waves'."
-- Erwin Schrödinger in Letter to John Lighton Synge (1959)
  1. Overview
  2. FID and FT
  3. Steps Before FT: Window Function and Zero Filling
  4. Truncation Artifacts from Digital Overflow
  5. Signal Folding or Aliasing
  6. Phase Correction: Zero Order (PH0) and First Order (PH1)
  7. Magnetization and Coherence
  8. Single Quantum Coherence, J-coupling
  9. Through-bond Coupling Among More Spins
  10. Coherence Transfer: Iz to IzSy
  11. 2D NMR: Create the Second Dimension
  12. Chemical Exchange Studies
  13. Nonequilibrium Exchange
  14. NOESY: Cross-Correlation Through Space
  15. Transient NOESY: A Better Method of Meauring NOEs
  16. Pulsed Field Gradients (PFG): Now You See It, Now You Don't
  17. DOSY: Diffusion Ordered Spectroscopy
  18. Gradient Shimming

Other Materials

  1. Quantitative NMR: Optimizing SNR and Integral Accuracy
  2. Chemical Shift Referencing on the Unified Scale
Updated, Jan-Feb 2019, Hongjun Zhou

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