2D 1H-13C HSQC

2D Gradient Enhanced 1H-13C HSQC

The following figure is the Varian gradient selected 2D 1H-13C HSQC pulse sequence (gHSQC) with the main parameters displayed (NMR500).

gHSQC sequence

Procedure (on NMR500)

Default parameters

The default setting (modified from Varian's default values) in our parameter set uses the following for a quick HSQC experiment:

Data Processing

You can use vnmrJ to process the data, but vnmrJ's graphics display for 2D spectrum leaves much to be desired. A separate program is strongly recommended for detailed processing and analysis.

One of the most widely used free processing/display software package for multi-dimensional NMR data processing is nmrPipe/nmrDraw. This program package has been installed on NMR500 and the data station. Details on how to use them will be updated.

Example spectrum collected on NMR500

  • Sample: Strychnine at ~ 25mg/mL (~ 100mM) in cdcl3
  • Data collected October 2010 (~12 mins with nt=2)
  • Processed and displayed with nmrPipe/nmrDraw
  • 1H multiplicity detection turned on: positive (black) peaks - CH2; negative (red) peaks: CH/CH3


Strychnine 1H-13C HSQC

Expansion of the upper right region:

1H-13C HSQC expansion

H.Zhou updated Oct 2010